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KMID : 0604220060130020035
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Korean Journal Investigative Dermatology
2006 Volume.13 No. 2 p.35 ~ p.42
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Cytokine Expression in Infrared and Ultraviollet Irradiated Artificial Skin
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Lee Jong-Hee
Lee Seung-Ho
Yoon Ho-Joon
Kim Ji-Eun
Cho Kwang-Hyun
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Abstract
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Human skin is exposed to significant amounts of infrared irradiation (IR). However, IR is known to have only biostimulant effect, thus the effects of IR on human skin have not been fully investigated. In this study, the immunological roles of ultraviolet radiation (UVR) and IR was examined in the view of the cytokine modulation using artificial skin. Artificial skin was made by 3-dimensional cultures of keratinocytes on dermal substitutes composed with fibroblasts and collagen gel. Noncytotoxic dose of UVA, UVB and IR was irradiated onto the manufactured artificial skin and 24 hours after, the morphological disturbances and the expression levels of the cytokine (IL-1α, IL-10 and TNF-α) in the irradiated artificial skin were observed. UVA preirradiation protected epidermis from sunburn cell formation caused by UVB exposure. Microscopic observation showed that IR exerted more influence on dermal collagen structures than epidermis. After UVA irradiation, IL-10 secretion from artificial skin increased and TNF-α, to the less degree. However, UVA irradiation itself seemed to fail to evoke IL-1α responses in the epidermis. UVB irradiation induced releases of all the cytokines examined. UVA preirradiation decreased IL-1α and IL-10 expression levels by over 50% evoked by UVB exposure. On the contrary, TNF-α release was augmented by UVA preirradiation before UVB exposure. IR increased expression levels of all the cytokines in a dose-dependent manner, especially IL-1α. In conclusion, UVA is thought to have effect on the modulation of the changes induced by UVB irradiation and IR itself plays significant role in the cytokine expression. More studies about mechanisms of immunological modulation and the interrelative effects of solar irradiation should be studied further.
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KEYWORD
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Infrared radiation, Ultraviolet radiation, Artificial skin, Cytokine
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